Introduction to the anatomy of a fluorescence microscope and the physical principles of its operation. Confocal and multi-photon microscopy. Molecular imaging applications based on Forster Resonance Energy Transfer (FRET), Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), Fluorescence Recovery After Photobleaching (FRAP) and Total Internal Reflection Fluorescence (TIRF) Microscopy.