Introduction to the anatomy of fluorescence microscopy and the physical principles of its operation; confocal and multi-photon microscopy; molecular imaging applications based on Forster Resonance Energy Transfer (FRET), Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), Fluorescence Recovery After Photobleaching (FRAP) and Total Internal Reflection Fluorescence (TIRF) Microscopy. Preferred background: basic understanding of general physics, general chemistry, general biology and basic calculus. Prerequisite: Accepted into the BE Professional Program or consent of the BE undergraduate advisor.